ArtNr |
ABC-AO1831a |
Hersteller |
Abcepta
|
Menge |
100 ul |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, FC, IHC, ELISA |
Clon |
4H9A5 |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CD166, ALCAM, MEMD, CD166 antigen, Activated leukocyte cell adhesion molecule |
Lieferbar |
|
Primary Accession |
Q13740 |
Antigen Type |
Recombinant Protein |
Application |
WB, IHC, FC, E |
Bio Background |
This gene encodes a C2H2 zinc finger protein with transactivation and DNA-binding activities. It has been shown to have anti-proliferative properties, and thus thought to function as a tumor suppressor. In addition, overexpression of this gene during fetal development is believed to underlie the rare disorder, transient neonatal diabetes mellitus (TNDM). This gene is imprinted, with preferential expression of the paternal allele in many tissues, however, biallelic expression has been noted in peripheral blood leucocytes. A recent study reports that tissue-specific imprinting results from variable utilization of monoallelic and biallelic promoters. Many transcript variants differing in the 5' UTR and encoding two different isoforms, have been found for this gene. ; |
Bio References |
1. Vascul Pharmacol. 2011 Mar-Jun; 54(3-6):93-9. 2. Int J Gynecol Cancer. 2011 Apr; 21(3):523-8. |
Clonality |
Monoclonal |
Gene ID |
214 |
Gene Name |
ALCAM |
Subtitle |
Purified Mouse Monoclonal Antibody |
Reactivity |
H, M |
Legend image 1 |
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng); |
Type image 1 |
E |
Dilution image 1 |
1/10000 |
Legend image 2 |
Figure 1: Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa) |
Type image 2 |
WB |
Dilution image 2 |
1/500 - 1/2000 |
Legend image 3 |
Figure 2: Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate. |
Type image 3 |
WB |
Dilution image 3 |
1/500 - 1/2000 |
Legend image 4 |
Figure 3: Western blot analysis using ALCAM mouse mAb against NIH/3T3 cell lysate. |
Type image 4 |
WB |
Dilution image 4 |
1/500 - 1/2000 |
Legend image 5 |
Figure 4: Flow cytometric analysis of Jurkat cells using ALCAM mouse mAb (green) and negative control (red). |
Type image 5 |
FC |
Dilution image 5 |
1/200 - 1/400 |
Legend image 6 |
Figure 5: Immunohistochemical analysis of paraffin-embedded prostate cancer tissues using ALCAM mouse mAb with DAB staining. |
Type image 6 |
IHC |
Dilution image 6 |
1/200 - 1/1000 |
Legend image 7 |
Figure 6: Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALCAM mouse mAb with DAB staining. |
Type image 7 |
IHC |
Dilution image 7 |
1/200 - 1/1000 |
Isotype |
IgG2b |
Calculated MW |
65102 |
Formulation |
Purified antibody in PBS with 0.05% sodium azide |
Description |
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. |
|
Clone: 4H9A5 |
Immunogen |
Purified recombinant fragment of human ALCAM (AA: 48-216) expressed in E. Coli. |
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