RAF1 Antibody

Recombinant Protein

1. Am J Hum Genet. 2009 Nov; 85(5):628-42. 2. Mol Cancer Res. 2009 Oct; 7(10):1635-44.

5894

Purified Mouse Monoclonal Antibody

Black line: Control Antigen (100 ng);
Purple line: Antigen(10ng);
Blue line: Antigen (50 ng);
Red line: Antigen (100 ng);

1/10000

WB

Figure 2: Western blot analysis using RAF1 mouse mAb against Hela (1), A431 (2), Cos7 (3) and C6 (4) cell lysate.

1/500 - 1/2000

IHC

Figure 4: Flow cytometric analysis of Hela cells using RAF1 mouse mAb (green) and negative control (red).

1/200 - 1/400

73052

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Purified recombinant fragment of human RAF1 expressed in E. Coli.