ArtNr |
ABC-AM8673b-ev |
Hersteller |
Abcepta
|
Menge |
50 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS. |
Applikationen |
WB |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
Proline-rich AKT1 substrate 1, 40 kDa proline-rich AKT substrate, AKT1S1 {ECO:0000312|EMBL:AAH16043.1} |
Lieferbar |
|
Primary Accession |
Q96B36 |
Antigen Type |
Recombinant Protein |
Application |
WB |
Bio Background |
Subunit of mTORC1, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, AKT1S1 negatively regulates mTOR activity in a manner that is dependent on its phosphorylation state and binding to 14-3-3 proteins. Inhibits RHEB-GTP-dependent mTORC1 activation. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. May also play a role in nerve growth factor-mediated neuroprotection. |
Bio References |
Ota T., et al.Nat. Genet. 36:40-45(2004).Totoki Y., et al.Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.Grimwood J., et al.Nature 428:529-535(2004).Mural R.J., et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.Kovacina K.S., et al.J. Biol. Chem. 278:10189-10194(2003). |
Clonality |
monoclonal |
Clone/Animal Names |
1997CT730.74.73 |
Gene ID |
84335 |
Gene Name |
AKT1S1 {ECO:0000312|EMBL:AAH16043.1} |
Subtitle |
Purified Mouse Monoclonal Antibody (Mab) |
Reactivity |
H, M |
Legend image 1 |
All lanes : Anti-AKT1S1 Antibody at 1:500-1:4000 dilutionLane 1: 293 whole cell lysateLane 2: Hela whole cell lysateLane 3: MCF-7 whole cell lysateLane 4: NIH/3T3 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:500-1:4000 |
Predicted |
H, M |
Isotype |
IgG1, κ |
Calculated MW |
27383 |
Antigen Source |
HUMAN |
Target/Specificity |
This AKT1S1 antibody is generated from a mouse immunized with a recombinant protein from the human region of human AKT1S1. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.