H2AFX Antibody (C-term)

Synthetic Peptide

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Monoclonal

3014

Mouse Monoclonal Antibody (Mab)

Western blot analysis of lysates from 293, CEM, HepG2, Jurkat, Hela, Raji cell line(from left to right). using H2AFX Antibody (C-term)(Cat. #AM2199b). AM2199b was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35μg per lane.

1:1000

IHC

Immunohistochemical analysis of paraffin-embedded H. prostate section using H2AFX Antibody (C-term)(Cat#AM2199b). AM2199b was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

1:25

IHC-P

IgG1

115-143

NP_002096.1