FASN Antibody (Center)

P49327

WB, IF

References for protein:
1.Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
2.Nguyen, P.L., et al. J. Clin. Oncol. 28(25):3958-3964(2010)
3.Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
4.Tischler, V., et al. Histopathology 56(6):811-815(2010)
5.Dorn, C., et al. Int J Clin Exp Pathol 3(5):505-514(2010)
References for HepG2 cell line:
1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960].
2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851].
3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761–1775. [PubMed:7506266].
4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1–3): 329–340. [PubMed:15138059].

497CT15.2.5

FASN

H

WB

Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2067b FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710).

1:200

IF

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FASN with AM2067b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm HepG2 cell line. The nuclear counter stain is DAPI (blue).

1:25

WB

All lanes : Anti-FASN Antibody (Center) at 1:8000 dilution
Lane 1: A549 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: 293 whole cell lysate
Lane 4: Ramos whole cell lysate
Lane 5: HepG2 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 273 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:8000

WB

NP_004095.4

273427

HUMAN

NP_004095.4