ArtNr |
ABC-AM2067B |
Hersteller |
Abcepta
|
Menge |
400 ul |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Applikationen |
WB, IF |
Specific against |
other |
Host |
Mouse |
Isotype |
IgG1 kappa |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
FAS, FASN, Fatty acid synthase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, 3-oxoacyl-[acyl-carrier-protein] synthase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, Enoyl-[acyl-carrier-protein] reductase, Oleoyl-[acyl-carrier-protein] hydrolase |
Lieferbar |
|
Primary Accession |
P49327 |
Antigen Type |
Synthetic Peptide |
Application |
WB, IF |
Bio Background |
The enzyme encoded by this gene is a multifunctionalprotein. Its main function is to catalyze the synthesis ofpalmitate from acetyl-CoA and malonyl-CoA, in the presence ofNADPH, into long-chain saturated fatty acids. In some cancer celllines, this protein has been found to be fused with estrogenreceptor-alpha (ER-alpha), in which the N-terminus of FAS is fusedin-frame with the C-terminus of ER-alpha. |
Bio References |
References for protein:1.Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)2.Nguyen, P.L., et al. J. Clin. Oncol. 28(25):3958-3964(2010)3.Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)4.Tischler, V., et al. Histopathology 56(6):811-815(2010)5.Dorn, C., et al. Int J Clin Exp Pathol 3(5):505-514(2010)References for HepG2 cell line:1. Knowles BB, et al. (1980). Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499.[ PubMed: 6248960].2. Darlington GJ, et al. (1987). Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354.[PubMed: 3034851].3. Ihrke, G; Neufeld, EB; Meads, T; Shanks, MR; Cassio, D; Laurent, M; Schroer, TA; Pagano, RE et al. (1993). "WIF-B cells: an in vitro model for studies of hepatocyte polarity". Journal of Cell Biology 123 (6): 1761–1775. [PubMed:7506266]. 4. Mersch-Sundermann, V.; Knasmüller, S.; Wu, X. J.; Darroudi, F.; Kassie, F. (2004). "Use of a human-derived liver cell line for the detection of cytoprotective, antigenotoxic and cogenotoxic agents". Toxicology 198 (1–3): 329–340. [PubMed:15138059]. |
Clonality |
Monoclonal |
Clone/Animal Names |
497CT15.2.5 |
Gene ID |
2194 |
Gene Name |
FASN |
Subtitle |
Mouse Monoclonal Antibody (Mab) |
Reactivity |
H |
Legend image 1 |
All lanes : Anti-FASN Antibody (Center) at 1:500-1:2000 dilutionLane 1: A549 whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: Ramos whole cell lysateLane 5: HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 1 |
WB |
Dilution image 1 |
1:500-1:2000 |
Legend image 2 |
Fluorescent confocal image of HepG2 cells stained with FASN (Center) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2067b FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the FASN immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000169710). |
Type image 2 |
IF |
Dilution image 2 |
1:200 |
Legend image 3 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS ((human cervical epithelial adenocarcinoma cell line) cells labeling FASN with AM2067b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm Hela cell line. The nuclear counter stain is DAPI (blue). |
Type image 3 |
IF |
Dilution image 3 |
1:25 |
Legend image 4 |
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FASN with AM2067b at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm HepG2 cell line. The nuclear counter stain is DAPI (blue). |
Type image 4 |
IF |
Dilution image 4 |
1:25 |
Legend image 5 |
FASN Antibody (Center) (Cat. #AM2067b) western blot analysis in mouse brain tissue lysates (35μg/lane).This demonstrates the FASN (Center) antibody detected the FASN (Center) protein (arrow). |
Type image 5 |
WB |
Dilution image 5 |
1:100 |
Legend image 6 |
All lanes : Anti-FASN Antibody (Center) at 1:8000 dilutionLane 1: A549 whole cell lysateLane 2: Hela whole cell lysateLane 3: 293 whole cell lysateLane 4: Ramos whole cell lysateLane 5: HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 6 |
WB |
Dilution image 6 |
1:8000 |
Legend image 7 |
Anti- at 1:1000 dilution + HepG2 whole cell lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 273 kDaBlocking/Dilution buffer: 5% NFDM/TBST. |
Type image 7 |
WB |
Dilution image 7 |
1:1000 |
Other Accession |
NP_004095.4 |
Isotype |
IgG1, κ |
Calculated MW |
273427 |
Antigen Region |
942-973 |
Antigen Source |
HUMAN |
Target/Specificity |
This FASN antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 942-973 amino acids from the Central region of human FASN. |
NCBI Accession |
NP_004095.4 |
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