« Zurück
Home /
Beta-Actin Antibody

Beta-Actin Antibody

ArtNr	ABC-AM1829B-ev
Hersteller	Abcepta
Menge	80 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applikationen	WB, FC, IF, IHC-P
Specific against	other
Host	Mouse
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	Actin, Beta-actin, ACTB, cytoplasmic 1, cytoplasmic 1, N-terminally processed
Lieferbar
Primary Accession	P60709
Antigen Type	Recombinant Protein
Application	WB, FC, IF, IHC-P
Bio Background	This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
Bio References	Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor alpha in human breast cancer cell nuclei. Ambrosino C, et al. Mol Cell Proteomics, 2010 Jun. PMID 20308691. Contribution of rearranged actin structures to the spread of Ectromelia virus infection in vitro. Boratynska A, et al. Acta Virol, 2010. PMID 20201613. Molecular mechanisms underlying nucleocytoplasmic shuttling of actinin-4. Kumeta M, et al. J Cell Sci, 2010 Apr 1. PMID 20197409. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004.
Clonality	Monoclonal
Clone/Animal Names	137CT26.1.1
Gene ID	60
Gene Name	ACTB
Subtitle	Mouse Monoclonal Antibody (Mab)
Reactivity	H, M, Rat
Legend image 1	Confocal immunofluorescent analysis of ACTB Antibody (Cat#AM1829b) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Type image 1	IF
Dilution image 1	1:10~50
Legend image 2	All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of anti-ACTB Antibody (Cat. #AM1829b) in K562, HL-60, Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35μg/lane). ACTB (arrow) was detected using the purified Mab.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	AM1829b staining ACTB in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Type image 5	IHC-P
Dilution image 5	1:25
Legend image 6	Immunohistochemical analysis of paraffin-embedded H.skeletal muscle section using Beta-Actin Antibody(Cat#AM1829b). AM1829b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 6	IHC-P
Dilution image 6	1:10~50
Legend image 7	Immunohistochemical analysis of paraffin-embedded H.spleen section using Beta-Actin Antibody(Cat#AM1829b). AM1829b was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 7	IHC-P
Dilution image 7	1:25
Other Accession	A2BDB0; P63259; P63260; P63261; Q5ZMQ2; P63258; P60711; Q6QAQ1; P60710; Q4R561; P60706; P60712; P53505; P60708; P60713
Predicted	X, B, C, Hs, Pr, Pig, Sh
Isotype	IgG1, Igk
Calculated MW	41737
Antigen Source	HUMAN
Target/Specificity	This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.
NCBI Accession	NP_001092.1

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Arbeitsanleitung (Data Sheet) anfordern

ABC-AM1829B-ev-datasheet.pdf

Sicherheitsdatenblatt anfordern

Einfache Bestellung Periodische Bestellung

Menge: 80 ul

lieferbar

Lieferung vsl. bis 06.09.2024

Express-Lieferung: 7 Tage sparen

Vergleichen

Auf den Wunschzettel

Weiterempfehlen per Mail