ArtNr |
ABC-AE1009b |
Hersteller |
Abcepta
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody Primary |
Format |
affinity Purified IgG, in PBS, 0.02% sodium azide and 50% glycerol. |
Applikationen |
WB, IF, IHC |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CFL1, p18, Cofilin, CFL, Cofilin-1, 18 kDa phosphoprotein, non-muscle isoform |
Lieferbar |
|
Primary Accession |
P23528 |
Application |
WB, IHC, IF |
Bio Background |
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. |
Bio References |
Actin-depolymerizing factor cofilin-1 is necessary in maintaining mature podocyte architecture. Garg P, et al. J Biol Chem, 2010 Jul 16. PMID 20472933. Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 May 14. PMID 20471030. Sex-specific proteome differences in the anterior cingulate cortex of schizophrenia. Martins-de-Souza D, et al. J Psychiatr Res, 2010 Apr 8. PMID 20381070. HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1. Quintela-Fandino M, et al. Proc Natl Acad Sci U S A, 2010 Feb 9. PMID 20133759. Tyrosine phosphorylation of cofilin at Y68 by v-Src leads to its degradation through ubiquitin-proteasome pathway. Yoo Y, et al. Oncogene, 2010 Jan 14. PMID 19802004. |
Clonality |
Polyclonal |
Gene ID |
1072 |
Gene Name |
CFL1 |
Subtitle |
Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H, M, Rat |
Legend image 1 |
Western blot analysis of extracts from COLO205 cells using Cofilin Phospho-Cofilin-S3 Antibody (Cat#AE1009b)(Lane 2)and the same antibody preincubated with blocking peptide(Lane 1). |
Type image 1 |
WB |
Dilution image 1 |
1:500~1:1000 |
Legend image 2 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Cofilin-S3 Antibody (Cat#AE1009b). |
Type image 2 |
IHC |
Dilution image 2 |
1:50~1:100 |
Legend image 3 |
Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-Cofilin-S3 Antibody |
Type image 3 |
IF |
Dilution image 3 |
1:100~200 |
Isotype |
Rabbit IgG |
Target/Specificity |
The antibody was affinity-purified from rabbit antiserum using epitope-specific phosphopeptide column, and the antibody against non-phosphopeptide was removed using non-phosphopeptide column corresponding to the phosphorylation site. |
NCBI Accession |
NP_005498.1 |
Concentration (mg/ml) |
1mg/ml |
Sequence |
Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V), according to the protein. NP_005498.1 |
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