Pol II monoclonal antibody - Classic

CHIP

5430

Purified mouse monoclonal Antibody

ChIP assays were performed using human HeLa cells, the Diagenode monoclonal antibody against Pol II (cat. No. ADN10297) and optimized PCR primer pairs for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010051), using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 µl of antibody per ChIP experiment was analyzed. IgG (2 µg/ IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the GAPDH and EIF4A2 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

2 µl/ChIP

WB