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H3K27ac polyclonal antibody

H3K27ac polyclonal antibody

ArtNr	ABC-ADN10256
Hersteller	Abcepta
Menge	50 ug
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Applikationen	WB, IF, ELISA, CHIP, Dot
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HIST1H3A, H3FA, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Lieferbar
Primary Accession	P68431
Application	WB, IF, E, DB, CHIP
Clonality	Polyclonal
Gene ID	8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968
Gene Name	H3C1 (HGNC:4766)
Subtitle	Purified rabbit Polyclonal Antibody
Reactivity	H, M
Legend image 1	To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody against H3K27ac (Cat. No. ADN10256). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8, 300.
Type image 1	E
Dilution image 1	1:500
Legend image 2	To test the cross reactivity of the Diagenode antibody against H3K27ac (Cat. No. ADN10256), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K27. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20, 000. Figure 4 shows a high specificity of the antibody for the modification of interest.
Type image 2	DB
Dilution image 2	1:20000
Legend image 3	Western blot was performed on whole cell (25 µg, lane 1) and histone extracts (15 µg, lane 2) from HeLa cells, and on 1 µg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Diagenode antibody against H3K27ac (Cat. No. C1541196). The antibody was diluted 1:1, 000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	HeLa cells were stained with the Diagenode antibody against H3K27ac (Cat. No. ADN10256) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K27ac antibody (top) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown at the bottom.
Type image 4	IF
Dilution image 4	1:500

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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ABC-ADN10256-datasheet.pdf

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Einfache Bestellung Periodische Bestellung

Menge: 50 ug

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Lieferung vsl. bis 08.11.2024

Express-Lieferung: 31 Tage sparen

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