ArtNr |
ABC-ADN10256 |
Hersteller |
Abcepta
|
Menge |
50 ug |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, IF, ELISA, CHIP, Dot |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
HIST1H3A, H3FA, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l |
Lieferbar |
|
Primary Accession |
P68431 |
Application |
WB, IF, E, DB, CHIP |
Clonality |
Polyclonal |
Gene ID |
8350; 8351; 8352; 8353; 8354; 8355; 8356; 8357; 8358; 8968 |
Gene Name |
|
Subtitle |
Purified rabbit Polyclonal Antibody |
Reactivity |
H, M |
Legend image 1 |
To determine the titer of the antibody, an ELISA was performed using a serial dilution of the Diagenode antibody against H3K27ac (Cat. No. ADN10256). The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:8, 300. |
Type image 1 |
E |
Dilution image 1 |
1:500 |
Legend image 2 |
To test the cross reactivity of the Diagenode antibody against H3K27ac (Cat. No. ADN10256), a Dot Blot analysis was performed with peptides containing other histone modifications and the unmodified H3K27. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20, 000. Figure 4 shows a high specificity of the antibody for the modification of interest. |
Type image 2 |
DB |
Dilution image 2 |
1:20000 |
Legend image 3 |
Western blot was performed on whole cell (25 µg, lane 1) and histone extracts (15 µg, lane 2) from HeLa cells, and on 1 µg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Diagenode antibody against H3K27ac (Cat. No. C1541196). The antibody was diluted 1:1, 000 in TBS-Tween containing 5% skimmed milk. The marker (in kDa) is shown on the left. |
Type image 3 |
WB |
Dilution image 3 |
1:1000 |
Legend image 4 |
HeLa cells were stained with the Diagenode antibody against H3K27ac (Cat. No. ADN10256) and with DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/ TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K27ac antibody (top) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown at the bottom. |
Type image 4 |
IF |
Dilution image 4 |
1:500 |
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