Anti-Cyclin D1 Picoband Antibody

WB

595

Anti- Cyclin D1 Picoband antibody, ABO12061, Western blottingAll lanes: Anti Cyclin D1 (ABO12061) at 0.5ug/mlLane 1: Human Placenta Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: 293T Whole Cell Lysate at 40ugPredicted bind size: 33KDObserved bind size: 33KD

0.1-0.5 µg/ml

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner. .

G1/S-specific cyclin-D1

A synthetic peptide corresponding to a sequence in the middle region of human CCND1(158-184aa HDFIEHFLSKMPEAEENKQIIRKHAQT), different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

No cross reactivity with other proteins.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.