ArtNr |
ABC-ABO11115 |
Hersteller |
Abcepta
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Lyophilized |
Applikationen |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
PCGF4, BMI1, RNF51, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51 |
Lieferbar |
|
Primary Accession |
P35226 |
Application |
WB |
Clonality |
Polyclonal |
Gene ID |
100532731; 648 |
Reactivity |
H |
Legend image 1 |
Anti-Bmi1 antibody, ABO11115, Western blottingAll lanes: Anti Bmi1 (ABO11115) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: HT1080 Whole Cell Lysate at 40ugLane 3: COLO320 Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 37KDObserved bind size: 37KD |
Type image 1 |
WB |
Dilution image 1 |
0.1-0.5 µg/ml |
Calculated MW |
36949 |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Description |
Rabbit IgG polyclonal antibody for Polycomb complex protein BMI-1(BMI1) detection. Tested with WB in Human. |
Protein Function |
Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. . |
Subcellular Localization |
Nucleus. Cytoplasm. |
Protein Name |
Polycomb complex protein BMI-1 |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
Immunogen |
A synthetic peptide corresponding to a sequence in the middle region of human Bmi1(136-152aa EFFDQNRLDRKVNKDKE), different from the related mouse sequence by three amino acids. |
Purification |
Immunogen affinity purified. |
Cross Reactivity |
No cross reactivity with other proteins |
Storage |
At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing. |
Concentration (mg/ml) |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Sequence Similarities |
Contains 1 RING-type zinc finger. |
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