Anti-Bag1 Picoband Antibody

WB, IHC-P

573

Figure 1. Western blot analysis of Bag1 using anti-Bag1 antibody (ABO10248). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bag1 antigen affinity purified polyclonal antibody (Catalog # ABO10248) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bag1 at approximately 39KD. The expected band size for Bag1 is at 39KD.

0.1-0.5 µg/ml

IHC

Figure 3. IHC analysis of Bag1 using anti-Bag1 antibody (ABO10248).Bag1 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Bag1 Antibody (ABO10248) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Figure 4. IHC analysis of Bag1 using anti-Bag1 antibody (ABO10248).Bag1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Bag1 Antibody (ABO10248) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

38779

Rabbit IgG polyclonal antibody for BAG family molecular chaperone regulator 1(BAG1) detection. Tested with WB, IHC-P in Human; Mouse; Rat.

Isoform 1: Nucleus. Cytoplasm. Isoform 1 localizes predominantly to the nucleus.

BAG family molecular chaperone regulator 1

A synthetic peptide corresponding to a sequence at the C-terminus of human Bag1 (323-345aa DTVEQNICQETERLQSTNFALAE), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.