ArtNr |
ABC-ABO10191 |
Hersteller |
Abcepta
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Lyophilized |
Applikationen |
WB |
Specific against |
other |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CA4, Carbonic anhydrase 4, Carbonate dehydratase IV, Carbonic anhydrase IV, CA-IV, 4.2.1.1 |
Lieferbar |
|
Primary Accession |
P22748 |
Application |
WB |
Clonality |
Polyclonal |
Gene ID |
762 |
Reactivity |
H, M, Rat |
Legend image 1 |
Figure 1. Western blot analysis of CA IV using anti- CA IV antibody (ABO10191). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CA IV antigen affinity purified polyclonal antibody (Catalog # ABO10191) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CA IV at approximately 35KD. The expected band size for CA IV is at 35KD. |
Type image 1 |
WB |
Dilution image 1 |
0.1-0.5 µg/ml |
Calculated MW |
35032 |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Description |
Rabbit IgG polyclonal antibody for Carbonic anhydrase 4(CA4) detection. Tested with WB in Human; Mouse; Rat. |
Protein Function |
Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. . |
Subcellular Localization |
Cell membrane; Lipid-anchor, GPI-anchor. |
Tissue Specificity |
Expressed in the endothelium of the choriocapillaris in eyes (at protein level). Not expressed in the retinal epithelium at detectable levels. . |
Protein Name |
Carbonic anhydrase 4 |
Contents |
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E.coli-derived human CA IV recombinant protein (Position: A19-S284). Human CA IV shares 55.5% and 58.9% amino acid (aa) sequence identity with mouse and rat CA IV, respectively. |
Purification |
Immunogen affinity purified. |
Cross Reactivity |
No cross reactivity with other proteins. |
Storage |
At -20?C for one year. After r?Constitution, at 4?C for one month. It?Can also be aliquotted and stored frozen at -20?C for a longer time.Avoid repeated freezing and thawing. |
Concentration (mg/ml) |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
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