Anti-Crp Picoband Antibody

WB, IHC-P, E

12944

Figure 1. Western blot analysis of Crp using anti-Crp antibody (ABO10045). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: rat thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Crp antigen affinity purified polyclonal antibody (Catalog # ABO10045) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Crp at approximately 25KD. The expected band size for Crp is at 25KD.

0.1-0.5 µg/ml

IHC

Figure 3. IHC analysis of Crp using anti- Crp antibody (ABO10045).Crp was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- Crp Antibody (ABO10045) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

25360

Rabbit IgG polyclonal antibody for C-reactive protein(Crp) detection. Tested with WB, IHC-P, ELISA in Mouse; Rat.

Secreted.

C-reactive protein

E. coli-derived mouse Crp recombinant protein (Position: H20-S225). Mouse Crp shares 71.9% and 72.7% amino acid (aa) sequence identity with human and rat Crp, respectively.

No cross reactivity with other proteins

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.