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Anti-Rat IP-10 Antibody

ArtNr ABC-ABG10317-U050
Hersteller Abcepta
Menge 50 ug
Kategorie
Typ Antibody Primary
Applikationen WB, IHC, ELISA
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Application
WB, IHC, E
Clonality
Polyclonal
Reactivity
Rat
Preparation
Produced from sera of rabbits immunized with highly pure recombinant Rat IP-10. Anti-Rat IP-10 specific antibody was purified by affinity chromatography employing an immobilized Rat IP-10 matrix.
WesternBlot
To detect Rat IP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IP-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich
To detect Rat IP-10 by sandwich ELISA (using 100μ l/well antibody solution) a concentration of 0.5 - 2.0 μ g/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Rat IP-10 (62-062BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IP-10.
Immunohistochemistry
This antibody stained colchicine injected rat brain (including the caudate putamen) tissue.  The primary antibody was incubated at 0.25 μ g/ml overnight at 4?C.  This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™ ) reagent.  Optimal concentrations and conditions may vary.    Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm.
Neutralization
To yield one-half maximal inhibition [ND50] of the biological activity of Rat IP-10 (100 ng/ml), a concentration of 5.0-10.0 μ g/ml of this antibody is required.
Formulation
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ug
Lieferbar: In stock
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