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Biotinylated Anti-Rat IL-6 Antibody

ArtNr ABC-ABG10298-U050
Hersteller Abcepta
Menge 50 ug
Kategorie
Typ Antibody Primary
Applikationen WB, ELISA
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Application
WB, E
Clonality
Polyclonal
Reactivity
Rat
Preparation
Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IL-6. Anti-Rat IL-6 specific antibody was purified by affinity chromatography and then biotinylated.
WesternBlot
To detect Rat IL-6 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µ g/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-6 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich
To detect Rat IL-6 by sandwich ELISA (using 100 μ l/well antibody solution) a concentration of 0.25 – 1.0 μ g/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Rat IL-6 (62-006P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-6.
Direct
To detect Rat IL-6 by direct ELISA (using 100 μ l/well antibody solution) a concentration of 0.25 – 1.0 μ g/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat IL-6.
Formulation
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 50 ug
Lieferbar: In stock
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