B-Cell Leukemia/Lymphoma 2 Human Recombinant (¿BH3)

Product Name: B-Cell Leukemia/Lymphoma 2 Human Recombinant (¿BH3)
Synonyms: Apoptosis regulator Bcl-2, BCL2, B-cell CLL/lymphoma 2, Bcl-2.
Description: Bcl-2 Des BH3 domain (93-107 residues) Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 203 amino acids 1-92 and 108-218.The Bcl-2 is expressed as His-Tag fusion protein and purified by proprietary chromatographic techniques.
Uniprot Accesion Number: P10415
Amino Acid Sequence:
Source: Escherichia Coli.
Physical Appearance and Stability: Lyophilized Bcl-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Bcl-2 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein contains 10mM Tris-HCL pH-8, 1mM EDTA and 250mM NaCl. Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Application: Input marker or positive control (Western Blotting).Function study.
Solubility: Suspend Bcl-2 in 100?l of 0.5M Acetic acid, over night at 4C. Dilute 10 fold into selected buffer system.BCL-2 has tendency to form intramolecular disulfide bond, 5mM DTT is recommended in assay buffer. When running SDS-PAGE gel, 10mM DTT is recommended.
Biological Activity:
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.