Description |
Product Name: Neurotrophin-3 Mouse Recombinant Synonyms: Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3, Neurotrophin-3, Ntf3, Ntf-3, AI316846, AI835689, Nt3. Description: Neurotrophin-3 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 119 amino acids and having a molecular mass of 13.6kDa. The NT-3 is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P20181 Amino Acid Sequence:YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT. Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution NGF-2 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: Lyophilized from 0.02% TFA. Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The activity, as determined by the dose-dependent proliferation of BaF3 cells transfected with the TrkB receptor, is typically in the range of 1-10 ng/ml, corresponding to a specific activity of 100, 000-1, 000, 000 units/mg. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |