Description |
Product Name: Prolactin Rat Recombinant Synonyms: Mammotropin, Luterotropic hormone, Lutetropin, PRL. Description: Prolactin Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 198 amino acids and having a molecular mass of 22.6 kDa. The Prolactin is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P01237 Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Val-Cys. Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Prolactin should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: The protein was lyophilized from a concentrated (1mg/ml) solution with 10mM sodium Phosphate buffer pH=8 and 50mM Nacl. Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized Prolactin in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2-11 was found to be < 0.065 ng/ml corresponding to a specific activity of 15, 400, 000 Units/mg. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |