Description |
Product Name: DLAT/DLST/BCOADC Recombinant Human Synonyms: Description: Recombinant antigen for solid (ELISA) and fluid phase diagnostic assays. Mixture of E2/dihydrolipamide acyltransferase-subunits from 3 mitochondrial protein complexes: pyruvate dehydrogenase complex (PDC-E2) having a molecular mass of 60, 630 Dalton (pI 5.8); 2-oxo-glutarate dehydrogenase complex (OGDC-E2) having a molecular mass of 42, 301 Dalton (pI 6.3); branched chain 2-oxo-acid dehydrogenase complex (BCOADCE2) having a molecular mass of 47, 321 Dalton (pI 6.5). Mixture contains equal mass of each protein component. cDNAs coding for the mature forms of the human PDC-E2, OGDC-E2 and BCOADC-E2 proteins individually fused to a hexa-histidine purification tag. Uniprot Accesion Number: Amino Acid Sequence: Source: Sf9 insect cells. Physical Appearance and Stability: Store at 4° C if entire vial will be used within 2-4 weeks. Store, frozen at -20° C for longer periods of time. Avoid multiple freeze-thaw cycles. Formulation and Purity: M2 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol. Greater than 75% as determined by SDS-PAGE. Application: Western blot with anti-M2-Antigen autoantibody-positive patient sera or monoclonal anti-hexa-His-tag antibody. Solubility: Biological Activity: Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |