Tumor Necrosis Factor-alpha Human Recombinant, Sf9

Product Name: Tumor Necrosis Factor-alpha Human Recombinant, Sf9
Synonyms: Tumor Necrosis Factor, TNFA, Tumor Necrosis Factor Ligand
Superfamily Member 2, Cachectin, TNF-Alpha, TNFSF2, TNF-A, Tumor Necrosis Factor
(TNF Superfamily, Member 2), Tumor Necrosis Factor-Alpha, TNF,
Macrophage-Derived, TNF, Monocyte-Derived, TNF Superfamily, APC1 Protein,
Member 2, DIF, Tumor necrosis factor.
Description: TNF a produced in Sf9 Baculovirus cells is a single,
glycosylated polypeptide chain containing 163 amino acids (77-233a.a.) and
having a molecular mass of 18.1kDa.  (Molecular size on SDS-PAGE will appear at
approximately 18-28kDa).
TNF a is expressed with a 6
amino acid His tag at C-Terminus and purified by proprietary chromatographic
techniques.
Uniprot Accesion Number: P01375
Amino Acid Sequence:VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV
PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL
GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIALHHH HHH.
Source: Sf9,
Baculovirus cells.
Physical Appearance and Stability: Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Formulation and Purity: TNF a protein solution (1mg/ml) contains Phosphate
Buffered Saline (pH 7.4) and 10% glycerol. Greater than 90.0% as determined bySDS-PAGE.
Application:
Solubility:
Biological Activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma
cells in the presence of the metabolic inhibitor actinomycin D. 
The ED50 for this effect is = 0.2 ng/ml.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.