Interleukin-12 Rat Recombinant

Product Name: Interleukin-12 Rat Recombinant
Synonyms: NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Description: Interleukin-12 Rat Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques.
Uniprot Accesion Number:
Amino Acid Sequence:The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.
Source: Baculovirus.
Physical Appearance and Stability: Lyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL12 should be stored at 4C between 2-7 days and for future use below -18C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Formulation and Purity: The protein (1mg/ml) was lyophilized with no additives. Greater than 95.0% as determined by SDS-PAGE.
Application:
Solubility: It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Biological Activity: ED50 range = 0.01-0.05 ng/ml corresponding to a specific activity of 20, 000, 000-100, 000, 000IU/mg as determined by the dose dependent secretion of IFN-gamma from PHA and IL-2 activated mouse splenocytes that have been costimulated with PMA. Optimal concentration for individual application should be determined by a dose response assay.
Shipping Format and Condition: Lyophilized powder at room temperature.
Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.