Description |
Goat anti-carbonic anhydrase XII (aa188-199), Biotinylated Antibody Principle Name: carbonic anhydrase XII (aa188-199), Biotinylated Official Symbol: CA12 All Name and Symbols: CA12; carbonic anhydrase 12; CA-XII; CAXII; HsT18816; T18816; carbonate dehydratase XII; carbonic anhydrase XII; carbonic dehydratase; tumor antigen HOM-RCC-3.1.3 Accession Number(s): NP_001209.1; NP_996808.1; NP_001280571.1 Peptide Sequence: CSHLQHVKYKGQE., is from internal region Human Gene ID(s): 771 Non-Human GeneID(s): Important Comments: This antibody is expected to recognize all reported isoforms (NP_001209.1; NP_996808.1; NP_001280571.1). Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB, IHC Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 128000. Western Blot: Approx 45kDa band observed in Human kidney lysates (calculated MW of 39.5kDa according to NP_001209.1). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3ug/ml. Reference(s): Muhammad E, Leventhal N, Parvari G, Hanukoglu A, Hanukoglu I, Chalifa-Caspi V, Feinstein Y, Weinbrand J, Jacoby H, Manor E, Nagar T, Beck JC, Sheffield VC, Hershkovitz E, Parvari R. Autosomal recessive hyponatremia due to isolated salt wasting in sweat associated with a mutation in the active site of Carbonic Anhydrase 12. Human genetics 2011 Apr 129 (4): 397-405..PMID: 21184099 |