Goat anti-ASC / TMS1, Biotinylated Antibody

Goat anti-ASC / TMS1, Biotinylated Antibody
Principle Name: ASC / TMS1, Biotinylated
Official Symbol: PYCARD
All Name and Symbols: PYCARD; PYD and CARD domain containing; ASC; CARD5; TMS; TMS-1; TMS1; caspase recruitment domain-containing protein 5; target of methylation-induced silencing 1
Accession Number(s): NP_037390.2; NP_660183.1
Peptide Sequence: CRESQSYLVEDLERS., is from C Terminus
Human Gene ID(s): 29108
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise both reported isoforms (NP_037390.2 and NP_660183.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 24kDa band observed in lysates of cell line U937 (calculated MW of 21.6kDa according to NP_037390.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3ug/ml.
Reference(s): Ohtsuka T, Ryu H, Minamishima YA, Macip S, Sagara J, Nakayama KI, Aaronson SA, Lee SW. ASC is a Bax adaptor and regulates the p53-Bax mitochondrial apoptosis pathway. Nature cell biology 2004 Feb 6 (2): 121-8..PMID: 14730312