Goat anti-E2F3 (mouse) Antibody

Goat anti-E2F3 (mouse) Antibody
Principle Name: E2F3 (mouse)
Official Symbol: E2f3
All Name and Symbols: E2f3; E2F transcription factor 3; E2F3b; E2f3a; mKIAA0075; OTTMUSP00000000663
Accession Number(s): NP_034223.1
Peptide Sequence: DLEKLPLVEDFMCS, is from C Terminus
Human Gene ID(s): 1871
Non-Human GeneID(s): 13557 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Mouse
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 60kDa and 48kDa band observed in Mouse fetal Lung lysates (calculated MW of 48.8kDa according to NP_034223.1). The 60kDa band was also observed in nuclear lysates of cell line NIH3T3 and it corresponds with different antibodies from
Reference(s): Wu L, Timmers C, Maiti B, Saavedra HI, Sang L, Chong GT, Nuckolls F, Giangrande P, Wright FA, Field SJ, Greenberg ME, Orkin S, Nevins JR, Robinson ML, Leone G, The E2F1-3 transcription factors are essential for cellular proliferation. Nature 2001 Nov 414 (6862): 457-62..PMID: 11719808