Goat anti-EAAT1 / GLAST1 Antibody

Goat anti-EAAT1 / GLAST1 Antibody
Principle Name: EAAT1 / GLAST1
Official Symbol: SLC1A3
All Name and Symbols: SLC1A3; solute carrier family 1 (glial high affinity glutamate transporter), member 3; EA6; EAAT1; FLJ25094; GLAST; GLAST1; GLAST-1; OTTHUMP00000116002; excitatory amino acid transporter 1; sodium-dependent glutamate/aspartate transporter 1; solute carrie
Accession Number(s): NP_004163.3; NP_001160167.1
Peptide Sequence: DNETEKPIDSETK, is from C Terminus
Human Gene ID(s): 6507
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 60kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration: 1-3ug/ml.
Reference(s): Vandenberg RJ, Handford CA, Campbell EM, Ryan RM, Yool AJ. Water and urea permeation pathways of the human excitatory amino acid transporter EAAT1. Biochem J. 2011 Oct 15; 439(2):333-40..PMID: 21732909