Goat anti-c-FOS (aa283-295) Antibody

Goat anti-c-FOS (aa283-295) Antibody
Principle Name: c-FOS (aa283-295)
Official Symbol: FOS
All Name and Symbols: FOS; FBJ murine osteosarcoma viral oncogene homolog; AP-1; C-FOS; p55; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); G0/G1 switch regulatory protein 7; activator protein 1; cellular oncogene c-fos; proto-oncogene c-Fos
Accession Number(s): NP_005243.1
Peptide Sequence: SETARSVPDMDLS, is from internal region
Human Gene ID(s): 2353
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 37kDa band observed in lysates of cell line HeLa (calculated MW of 40.7kDa according to NP_005243.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 5ug/ml.
Reference(s): Kang SM, Lim S, Won SJ, Shin YJ, Lim YS, Ahn BY, Hwang SB. c-Fos regulates hepatitis C virus propagation. FEBS Lett. 2011 Oct 20; 585(20):3236-44..PMID: 21920361