Goat anti-Gpx1 (mouse aa53-67) Antibody

Goat anti-Gpx1 (mouse aa53-67) Antibody
Principle Name: Gpx1 (mouse aa53-67)
Official Symbol: Gpx1
All Name and Symbols: Gpx1; glutathione peroxidase 1; AI195024; AL033363; CGPx; GPx-1; GSHPx-1; Gpx; cellular glutathione peroxidase
Accession Number(s): NP_032186.2
Peptide Sequence: DYTEMNDLQKRLGPR, is from internal region
Human Gene ID(s):
Non-Human GeneID(s): 14775 (mouse); 24404 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 22kDa band observed in Rat Kidney and Liver lysates and in Mouse Kidney lysates (calculated MW of 22.3kDa according to NP_032186.2). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Lubos E, Kelly NJ, Oldebeken SR, Leopold JA, Zhang YY, Loscalzo J, Handy DE. Glutathione peroxidase-1 deficiency augments proinflammatory cytokine induced redox signaling and human endothelial cell activation. J Biol Chem. 2011 Oct 14; 286(41):35407-17..PMID: 21852236