Goat anti-Doublecortin (mouse) Antibody

Goat anti-Doublecortin (mouse) Antibody
Principle Name: Doublecortin (mouse)
Official Symbol: Dcx
All Name and Symbols: Dcx; doublecortin; Dbct; OTTMUSP00000020394; OTTMUSP00000020395; OTTMUSP00000020396; OTTMUSP00000020397; doublecortex; doublin; lis-X; lissencephalin-X; lissencephaly, X-linked (doublecortin); neuronal migration protein doublecortin
Accession Number(s): NP_001103692.1; NP_001103694.1; NP_034155.2
Peptide Sequence: PKASPTPQKTSAKS, is from internal region
Human Gene ID(s): 1641
Non-Human GeneID(s): 13193 (mouse); 84394 (rat);
Important Comments: This antibody is expected to recognize all reported mouse isoforms (NP_001103692.1; NP_001103694.1; NP_034155.2). Reported variants represent identical protein: NP_001103692.1, NP_001103693.1
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IF
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 40kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to NP_001103692.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.
Reference(s): Klempin F, Kronenberg G, Cheung G, Kettenmann H, Kempermann G. Properties of doublecortin-(DCX)-expressing cells in the piriform cortex compared to the neurogenic dentate gyrus of adult mice. PLoS One. 2011; 6(10):e25760.PMID: 22022443