Description |
Goat anti-Doublecortin (mouse) Antibody Principle Name: Doublecortin (mouse) Official Symbol: Dcx All Name and Symbols: Dcx; doublecortin; Dbct; OTTMUSP00000020394; OTTMUSP00000020395; OTTMUSP00000020396; OTTMUSP00000020397; doublecortex; doublin; lis-X; lissencephalin-X; lissencephaly, X-linked (doublecortin); neuronal migration protein doublecortin Accession Number(s): NP_001103692.1; NP_001103694.1; NP_034155.2 Peptide Sequence: PKASPTPQKTSAKS, is from internal region Human Gene ID(s): 1641 Non-Human GeneID(s): 13193 (mouse); 84394 (rat); Important Comments: This antibody is expected to recognize all reported mouse isoforms (NP_001103692.1; NP_001103694.1; NP_034155.2). Reported variants represent identical protein: NP_001103692.1, NP_001103693.1 Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB, IF Tested Species: Human, Mouse Peptide ELISA: antibody detection limit dilution 1 to 128000. Western Blot: Approx 40kDa band observed in Mouse fetal Brain lysates (calculated MW of 40.6kDa according to NP_001103692.1). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour. Reference(s): Klempin F, Kronenberg G, Cheung G, Kettenmann H, Kempermann G. Properties of doublecortin-(DCX)-expressing cells in the piriform cortex compared to the neurogenic dentate gyrus of adult mice. PLoS One. 2011; 6(10):e25760..PMID: 22022443 |