Description |
Goat anti-Mucin 10 / Prol1 Antibody Principle Name: Mucin 10 / Prol1 Official Symbol: Prol1 All Name and Symbols: Prol1; proline rich, lacrimal 1; Muc10; mucin 10; mucin 10, submandibular gland salivary mucin Accession Number(s): NP_032670.2 Peptide Sequence: PKRQIISDKTKP, is from internal region Human Gene ID(s): Non-Human GeneID(s): 17830 (mouse); Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA Tested Species: Peptide ELISA: antibody detection limit dilution 1 to 8000. Western Blot: Preliminary experiments gave an approx 48kDa band in Mouse Eye lysates after 2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.6kDa a Reference(s): Wisner A, Dufour E, Messaoudi M, Nejdi A, Marcel A, Ungeheuer MN, Rougeot C, Human Opiorphin, a natural antinociceptive modulator of opioid-dependent pathways. Proceedings of the National Academy of Sciences of the United States of America 2006 Nov 103 (47): 17979-84..PMID: 17101991 |