Goat anti-CCP4 (aa748-761) Antibody

Goat anti-CCP4 (aa748-761) Antibody
Principle Name: CCP4 (aa748-761)
Official Symbol: AGBL1
All Name and Symbols: AGBL1; ATP/GTP binding protein-like 1; FLJ32310; MGC149239; ATP/GTP-binding protein-like 1; OTTHUMP00000195714; cytosolic carboxypeptidase 4, CCP4
Accession Number(s): NP_689549.2
Peptide Sequence: RPYQVITARVHPGE, is from internal region
Human Gene ID(s): 123624
Non-Human GeneID(s): 244071 (mouse); 308724 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Preliminary experiments gave bands at approx 100kDa and 24kDa in HeLa lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of
Reference(s): Rogowski K, van Dijk J, Magiera MM, Bosc C, Deloulme JC, Bosson A, Peris L, Gold ND, Lacroix B, Grau MB, Bec N, Larroque C, Desagher S, Holzer M, Andrieux A, Moutin MJ, Janke C. A family of protein-deglutamylating enzymes associated with
neurodegeneration. Cell. 2010 Nov 12; 143(4):564-78..PMID: 21074048