Goat anti-AKAP12 (mouse) Antibody

Goat anti-AKAP12 (mouse) Antibody
Principle Name: AKAP12 (mouse)
Official Symbol: Akap12
All Name and Symbols: Akap12; A kinase (PRKA) anchor protein (gravin) 12; AI317366; SSeCKS; Srcs5; Tsga12; A-kinase anchor protein 12; AKAP-12; gercelin; germ cell lineage protein gercelin; src-suppressed C kinase substrate; testis specific gene A12; v-src suppressed transcri
Accession Number(s): NP_112462.1
Peptide Sequence: SPDTNGPKLTEEGD, is from internal region
Human Gene ID(s):
Non-Human GeneID(s): 83397 (mouse); 83425 (rat);
Important Comments: This antibody is expected to recognize isoform 1 (NP_005091.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Preliminary experiments in lysates of cell lines NIH3T3 and of Mouse and Rat Brain and Testis gave no specific signal but low background (at antibody concentration up to 2ug/ml). We would appreciate any feedback from people in the field - h
Reference(s): Jo UH, Whang YM, Sung JS, Kim YH. Methylation of AKAP12{alpha} promoter in lung cancer. Anticancer Res. 2010 Nov; 30(11):4595-600..PMID: 21115911