Goat anti-NOX5 Antibody

Goat anti-NOX5 Antibody
Principle Name: NOX5
Official Symbol: NOX5
All Name and Symbols: NOX5; NADPH oxidase, EF-hand calcium binding domain 5; MGC149776; MGC149777; NADPH oxidase 5; OTTHUMP00000164443; OTTHUMP00000164444
Accession Number(s): NP_078781.3; NP_001171708.1; NP_001171709.1
Peptide Sequence: EWHPFTISSAPEQKD, is from internal region
Human Gene ID(s): 79400
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognize all reported isoforms (NP_078781.3; NP_001171708.1; NP_001171709.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 90kDa band observed in Human Testis lysates (calculated MW of 86.4kDa according to NP_078781.3). Recommended concentration: 0.5-2ug/ml.
Reference(s): Choi H, Kim S, Kim HJ, Kim KM, Lee CH, Shin JH, Noh M, Sphingosylphosphorylcholine down-regulates filaggrin gene transcription through NOX5-based NADPH oxidase and cyclooxygenase-2 in human keratinocytes. Biochemical pharmacology 2010 Jul 80 (1): 95-103..PMID: 20230798