Goat anti-Desmoplakin (aa 1672 -1684) Antibody

Pep ELISA, IHC

Goat anti-Desmoplakin (aa 1672 -1684) Antibody
Principle Name: Desmoplakin (aa 1672 -1684)
Official Symbol: DSP
All Name and Symbols: DSP; desmoplakin; DPI; 250kDa paraneoplastic pemphigus antigen; desmoplakin I
Accession Number(s): NP_004406.2
Peptide Sequence: QESVKQAHLRNEH, is from internal region
Human Gene ID(s): 1832
Non-Human GeneID(s): 109620 (mouse); 306871 (rat);
Important Comments: This antibody is expected to recognize reported isoform I (NP_004406.2) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been
Immunohistochemistry: In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6ug/ml.
Reference(s): Barahona-Dussault C, Benito B, Campuzano O, Iglesias A, Leung TL, Robb L, Talajic M, Brugada R, Role of genetic testing in arrhythmogenic right ventricular cardiomyopathy/dysplasia. Clinical genetics 2010 Jan 77 (1): 37-48.PMID: 19863551