Goat anti-TGFBI Antibody

Goat anti-TGFBI Antibody
Principle Name: TGFBI
Official Symbol: TGFBI
All Name and Symbols: TGFBI; transforming growth factor, beta-induced, 68kDa; BIGH3; CDB1; CDG2; CDGG1; CSD; CSD1; CSD2; CSD3; EBMD; LCD1; RGD-containing collagen-associated protein; kerato-epithelin
Accession Number(s): NP_000349.1
Peptide Sequence: QLYTDRTEKLRPE, is from internal region
Human Gene ID(s): 7045
Non-Human GeneID(s): 21810 (mouse); 24511 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 70kDa band observed in Human Kidney lysates (calculated MW of 74.7kDa according to Human NP_000349.1). Recommended concentration: 0.2-0.5ug/ml.
Immunohistochemistry: In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration: 4-6ug/ml.
Reference(s): Zenteno JC, Correa-Gomez V, Santacruz-Valdez C, Suarez-Sanchez R, Villanueva-Mendoza C, Clinical and genetic features of TGFBI-linked corneal dystrophies in Mexican population: description of novel mutations and novel genotype-phenotype correlations. Experimental eye research 2009 Aug 89 (2): 172-7..PMID: 19303004