Goat anti-RNF7 Antibody

Goat anti-RNF7 Antibody
Principle Name: RNF7
Official Symbol: RNF7
All Name and Symbols: RNF7; ring finger protein 7; CKBBP1; ROC2; SAG; OTTHUMP00000180645; regulator of cullins 2; sensitive to apoptosis, zinc RING finger protein SAG, regulator of cullins 2; zinc RING finger protein SAG
Accession Number(s): NP_055060.1
Peptide Sequence: DACLRCQAENKQE, is from internal region
Human Gene ID(s): 9616
Non-Human GeneID(s): 19823 (mouse);
Important Comments: This antibody is expected to recognize isoform 1 (NP_055060.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 11kDa band observed in Human Human Heart lysates (calculated MW of 12.7kDa according to NP_055060.1). Recommended concentration: 1-3ug/ml.
An additional band of 28kDa was consistently observed, however this band was not blocked by th
Reference(s): Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA, E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Molecular cell 2009 Feb 33 (4): 483-95..PMID: 19250909