Goat anti-Stromal antigen 2 / STAG2 Antibody

Goat anti-Stromal antigen 2 / STAG2 Antibody
Principle Name: Stromal antigen 2 / STAG2
Official Symbol: STAG2
All Name and Symbols: STAG2; stromal antigen 2; DKFZp686P168; DKFZp781H1753; FLJ25871; SA-2; SA2; bA517O1.1; OTTHUMP00000024338; OTTHUMP00000043514; OTTHUMP00000043515; OTTHUMP00000081754; OTTHUMP00000081755; SCC3 homolog 2
Accession Number(s): NP_001036214.1; NP_006594.3
Peptide Sequence: SRGSTVRSKKSKPST, is from internal region
Human Gene ID(s): 10735
Non-Human GeneID(s): 20843 (mouse); 313304 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001036214.1; NP_006594.3). Reported variants NP_001036215.1 and NP_001036214.1 represent identical protein: Reported variants NP_001036216.1 and NP_006594.3 represent identical protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human, Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 160kDa band observed in lysates of cell line K562 and ca.140kDa in rodent Spleen while ca.150kDa in Mouse Thymus lysates (calculated MW of 146kDa according to Human NP_001036214.1 and 140kDa according to Mouse NP_001071180.1). Recommen
Reference(s): Hauf S, Roitinger E, Koch B, Dittrich CM, Mechtler K, Peters JM, Dissociation of cohesin from chromosome arms and loss of arm cohesion during early mitosis depends on phosphorylation of SA2. PLoS biology 2005 Mar 3 (3): e69..PMID: 15737063