Description |
Goat anti-SIGLEC8 Antibody Principle Name: SIGLEC8 Official Symbol: SIGLEC8 All Name and Symbols: SIGLEC8; sialic acid binding Ig-like lectin 8; MGC59785; SAF2; SIGLEC-8; SIGLEC8L Accession Number(s): NP_055257.2 Peptide Sequence: SYKSQLNYKTKQ, is from internal region Human Gene ID(s): 27181 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Approx. 45kDa band observed in lysates of cell line MOLT4 (calculated MW of 54.0kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 J Reference(s): Yokoi H, Choi OH, Hubbard W, Lee HS, Canning BJ, Lee HH, Ryu SD, von Gunten S, Bickel CA, Hudson SA, Macglashan DW Jr, Bochner BS. Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement. J Allergy Clin Immunol. 2008 Feb; 121(2):499-505.e1. Epub 2007 Nov 26..PMID: 18036650 |