Goat anti-MSH5 Antibody

Goat anti-MSH5 Antibody
Principle Name: MSH5
Official Symbol: MSH5
All Name and Symbols: MSH5; mutS homolog 5 (E. coli) ; XXbac-BCX40G17.5; DKFZp434C1615; G7N: MGC2939; MutSH5; NG23; mutS homolog 5
Accession Number(s): NP_079535.3 ; NP_751897.1; NP_002432.1
Peptide Sequence: GDLHCEIRDQET, is from internal region
Human Gene ID(s): 4439
Non-Human GeneID(s): 17687 (mouse); 294252 (rat);
Important Comments: This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Preliminary experiments gave an approx 38kDa band in Human Testis, Tonsil and Thymus lysates after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc
Immunohistochemistry: In paraffin embedded Human Testis shows nuclear staining in spermatocytes. Recommended concentration, 2.5-3.8ug/ml.
Reference(s): Neyton S, Lespinasse F, Lahaye F, Staccini P, Paquis-Flucklinger V, Santucci-Darmanin S. CRM1-dependent nuclear export and dimerization with hMSH5 contribute to the regulation of hMSH4 subcellular localization. Exp Cell Res. 2007 Oct 15; 313(17):3680-93. Epub 2007 Aug 21..PMID: 17869244