Description |
Goat anti-COL4A3BP (aa662-672) Antibody Principle Name: COL4A3BP (aa662-672) Official Symbol: COL4A3BP All Name and Symbols: COL4A3BP; collagen, type IV, alpha 3 (Goodpasture antigen) binding protein ; CERT; CERTL; FLJ20597; GPBP ; STARD11; Goodpasture antigen-binding protein; START domain containing 11; alpha 3 type IV collagen binding protein; ceramide transporter; Accession Number(s): NP_005704.1; NP_112729.1 Peptide Sequence: DHDSAPLNNRC, is from internal region Human Gene ID(s): 10087 Non-Human GeneID(s): 68018 (mouse); Important Comments: This antibody is expected to recognise both reported isoforms (NP_005704.1 and NP_112729.1) Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Mouse Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: Approx 60kDa band observed in mouse heart lysates (calculated MW of 71.1kDa according to mouse NP_075909.1 and 60.0kDa according to human NP_112729.1). Recommended concentration: 0.5-1.5ug/ml. An additional band of unknown identity was als Reference(s): Swanton C, Marani M, Pardo O, Warne PH, Kelly G, Sahai E, Elustondo F, Chang J, Temple J, Ahmed AA, Brenton JD, Downward J, Nicke B. Regulators of mitotic arrest and ceramide metabolism are determinants of sensitivity to paclitaxel and other chemotherapeutic drugs. Cancer. 2007 Jun; 11(6):498-512..PMID: 17560332 |