Goat anti-Mammaglobin A / SCGB2A2 Antibody

Goat anti-Mammaglobin A / SCGB2A2 Antibody
Principle Name: Mammaglobin A / SCGB2A2
Official Symbol: SCGB2A2
All Name and Symbols: SCGB2A2; secretoglobin, family 2A, member 2 ; HGNC:7050; MGB1; UGB2; MGC71974; mammaglobin 1; mammaglobin A
Accession Number(s): NP_002402.1
Peptide Sequence: NPQVSKTEYKEL, is from internal region
Human Gene ID(s): 4250
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as small as the calculated size of 10.5kDa according to NP_002402.1. Therefore we cannot recommend an optimal concentration and the product i
Immunohistochemistry: In paraffin embedded Human Breast shows staining of cytoplasm in some acinar cells.. Recommended concentration, 4-6ug/ml.
Reference(s): Ouellette RJ, Richard D, Maicas E. RT-PCR for mammaglobin genes, MGB1 and MGB2, identifies breast cancer micrometastases in sentinel lymph nodes. Am J Clin Pathol. 2004 May; 121(5):637-43. Erratum in: Am J Clin Pathol. 2004 Nov; 122(5):813..PMID: 15151203