Goat anti-GLuR5 / GRIK1 Antibody

Goat anti-GLuR5 / GRIK1 Antibody
Principle Name: GLuR5 / GRIK1
Official Symbol: GRIK1
All Name and Symbols: GLUR5; GRIK1; glutamate receptor, ionotropic, kainate 1; EAA3 ; EEA3 ; GLR5 ; excitatory amino acid receptor 3
Accession Number(s): NP_000821.1; NP_783300.1
Peptide Sequence: QCKQTHPTNSTS, is from internal region (near the C Terminus)
Human Gene ID(s): 2897
Non-Human GeneID(s): 14805 (mouse); 29559 (rat);
Important Comments: This antibody is expected to recognise both reported isoforms according to NP_000821.1 and NP_783300.1.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 150kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 104kDa according to NP_000821.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (
Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 3.75ug/ml.
Reference(s): Martin S, Nishimune A, Mellor JR, Henley JM. SUMOylation regulates kainate-receptor-mediated synaptic transmission. Nature. 2007 May 17; 447(7142):321-5. Epub 2007 May 7..PMID: 17486098