Goat anti-Perilipin (internal) Antibody

Goat anti-Perilipin (internal) Antibody
Principle Name: Perilipin (internal)
Official Symbol: PLIN
All Name and Symbols: PLIN; perilipin; PERI; lipid droplet-associated protein
Accession Number(s): NP_002657.2
Peptide Sequence: PREKPKRRVSDS, is from internal region (near the C Terminus)
Human Gene ID(s): 5346
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 60kDa band observed in Human Adipose lysates (calculated MW of 56.0kDa according to NP_002657.2). Recommended concentration: 0.01 ug/ml. Primary incubation was 1 hour.
Immunohistochemistry: Paraffin embedded Human Colon. Recommended concentration: 2ug/ml.
Reference(s): Wolins NE, Brasaemle DL, Bickel PE. A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Lett. 2006 Oct 9; 580(23):5484-91. Epub 2006 Sep 1. Review. .PMID: 16962104