Goat anti-Biglycan Preproprotein Antibody

Goat anti-Biglycan Preproprotein Antibody
Principle Name: Biglycan Preproprotein
Official Symbol: BGN
All Name and Symbols: Biglycan Preproprotein; biglycan; BGN; DSPG1; PG-S1; PGI; SLRR1A ; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; small leucine-rich protein 1A
Accession Number(s): NP_001702.1
Peptide Sequence: DRLAIQFGNYKK, is from C Terminus
Human Gene ID(s): 633
Non-Human GeneID(s): 12111 (mouse); 25181 (rat);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Rat
Peptide ELISA: antibody detection limit dilution 1 to 4000.
Western Blot: Approx 37kDa band observed in Rat Skin lysates (calculated MW of 41.7kDa according to Human NP_001702.1 and Rat NP_058783.1). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Chen WB, Lenschow W, Tiede K, Fischer JW, Kalthoff H, Ungefroren H. Smad4/DPC4-dependent regulation of biglycan gene expression by transforming growth factor-beta in pancreatic tumor cells. J Biol Chem. 2002 Sep 27; 277(39):36118-28. Epub 2002 Jul 24. .PMID: 12140283