Goat anti-PIK3CA / P110alpha Antibody

Pep ELISA, IHC

Goat anti-PIK3CA / P110alpha Antibody
Principle Name: PIK3CA / P110alpha
Official Symbol: PIK3CA
All Name and Symbols: PIK3CA; p110-alpha; phosphoinositide-3-kinase, catalytic, alpha polypeptide ; MGC142161; MGC142163; PI3K; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalyti
Accession Number(s): NP_006209.2
Peptide Sequence: HQWLKDKNKGEIYD, is from internal region
Human Gene ID(s): 5290
Non-Human GeneID(s): 18706 (mouse); 170911 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1ug/ml).
Immunohistochemistry: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration: 2-3ug/ml. Paraffin embedded Human Heart. Recommended concentration: 5ug/ml.
Reference(s): Lopez-Knowles E, Hernandez S, Malats N, Kogevinas M, Lloreta J, Carrato A, Tardon A, Serra C, Real FX. PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors. Cancer Res. 2006 Aug 1; 66(15):7401-4. .PMID: 16885334