Description |
Goat anti-UCP1 Antibody Principle Name: UCP1 Official Symbol: UCP1 All Name and Symbols: UCP1; uncoupling protein 1 (mitochondrial, proton carrier); HGNC:12517; SLC25A7; UCP; mitochondrial brown fat uncoupling protein; uncoupling protein 1 Accession Number(s): NP_068605.1 Peptide Sequence: EQLKRELSKSRQ, is from C Terminus Human Gene ID(s): 7350 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA, WB Tested Species: Human Peptide ELISA: antibody detection limit dilution 1 to 128000. Western Blot: Approx 35kDa band observed in Human Adipose lysates (calculated MW of 33.0kDa according to NP_068605.1). Recommended concentration: 0.1-1 ug/ml. Primary incubation was 1 hour. Reference(s): Ishigaki Y, Katagiri H, Yamada T, Ogihara T, Imai J, Uno K, Hasegawa Y, Gao J, Ishihara H, Shimosegawa T, Sakoda H, Asano T, Oka Y. Dissipating excess energy stored in the liver is a potential treatment strategy for diabetes associated with obesity. Diabetes. 2005 Feb; 54(2):322-32. .PMID: 15677488 |