Goat anti-GAPDH (Internal) Antibody

Goat anti-GAPDH (Internal) Antibody
Principle Name: GAPDH (Internal)
Official Symbol: GAPDH
All Name and Symbols: GAPDH; glyceraldehyde-3-phosphate dehydrogenase; HGNC:4141; G3PD; GAPD; MGC88685; OTTHUMP00000174431; OTTHUMP00000174432; aging-associated gene 9 protein; glyceraldehyde 3-phosphate dehydrogenase
Accession Number(s): NP_002037.2; NP_001243728.1
Peptide Sequence: GVNHEKYDNSLK, is from internal region
Human Gene ID(s): 2597
Non-Human GeneID(s): 14433 (mouse); 24383 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002037.2; NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC, IF
Tested Species: Human, Mouse, Rat
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1
Immunohistochemistry: In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2ug/ml.
Reference(s): Ismail SA, Park HW. Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase. Acta Crystallogr D Biol Crystallogr. 2005 Nov; 61(Pt 11):1508-13. Epub 2005 Oct 19.PMID: 16239728