Goat anti-TRIM5 / RNF88 (alpha isoform) Antibody

Goat anti-TRIM5 / RNF88 (alpha isoform) Antibody
Principle Name: TRIM5 / RNF88 (alpha isoform)
Official Symbol: TRIM5
All Name and Symbols: TRIM5; tripartite motif-containing 5 ; HGNC:16276; RNF88; tripartite motif protein 5; tripartite motif protein TRIM5; TRIM5alpha; OTTHUMP00000069812; tripartite motif protein TRIM; tripartite motif-containing protein 5 alpha
Accession Number(s): NP_149023.1
Peptide Sequence: CNIEKNENYQPK, is from internal region
Human Gene ID(s): 85363
Non-Human GeneID(s):
Important Comments: This antibody is expected to recognise isoform alpha (NP_149023.1) only.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, IHC
Tested Species:
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe g
Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6ug/ml.
Reference(s): Berthoux L, Sebastian S, Sokolskaja E, Luban J. Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells. Proc Natl Acad Sci U S A. 2005 Oct 11; 102(41):14849-53. Epub 2005 Oct 3. .PMID: 16203999