Goat anti-SF3B4 / SAP49 Antibody

Goat anti-SF3B4 / SAP49 Antibody
Principle Name: SF3B4 / SAP49
Official Symbol: SF3B4
All Name and Symbols: SF3B4; SAP49; splicing factor 3b, subunit 4, 49kD; SF3B49; MGC10828; spliceosome-associated protein (U2 snRNP); spliceosomal protein; splicing factor 3b, subunit 4; SF3b49; pre-mRNA splicing factor SF3b 49 kDa subunit; splicing factor 3b, subunit 4
Accession Number(s): NP_005841.1
Peptide Sequence: AAGPISERNQDAT, is from N Terminus
Human Gene ID(s): 10262
Non-Human GeneID(s): 107701 (mouse);
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 48kDa band observed in Human HeLa lysates (calculated MW of 44.4kDa according to NP005841.1). Recommended concentration: 0.1-0.3ug/ml.
Reference(s): Champion-Arnaud P, Reed R. The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. Genes Dev 1994 Aug 15; 8(16):1974-83.PMID: 7958871