Description |
Goat anti-PEPP3 / PLEKHA6 Antibody Principle Name: PEPP3 / PLEKHA6 Official Symbol: PLEKHA6 All Name and Symbols: PLEKHA6; PEPP3; KIAA0969; phosphoinositol 3-phosphate-binding protein-3; pleckstrin homology domain containing, family A member 6; MGC176733; phosphoinositol 3-phosphate-binding protein 3 Accession Number(s): NP_055750.2 Peptide Sequence: SPRGADSSYTMRV, is from C Terminus Human Gene ID(s): 22874 Non-Human GeneID(s): Important Comments: Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. Applications: Pep ELISA Tested Species: Peptide ELISA: antibody detection limit dilution 1 to 32000. Western Blot: No signal obtained yet but a preliminary experiment showed low background in Human Heart extract at up to 1ug/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Reference(s): Dowler S, Currie RA, Campbell DG, Deak M, Kular G, Downes CP, Alessi DR. Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities. Biochem J. 2000 Oct 1; 351(Pt 1):19-31..PMID: 11001876 |